Thesis Number |
level |
Language |
Year |
Bilim Dalı |
|
Yüksek Lisans |
Türkçe |
2022 |
Diğer |
Several herbicide-tolerant crops have been developed and commercialized as a result of the advancement of plant biotechnology, allowing for high agricultural yields. The objective of this study was to determine imazamox (IMI) herbicide-resistant cumin (Cuminum cyminum L.) genotype through in vitro selection. The experiments were carried out in the Plant Biotechnology Laboratories of Agriculture Faculty, Erciyes University. Hypocotyls from the cumin plant were utilized as the source of explant for the tests, and were cultured on MS (Murashige and Skoog) medium supplemented with various concentrations and combinations of 2,4-D and BAP for callus induction and proliferation. The effect of different concentrations and combinations of 2,4-D and BAP (0.00, 0.50, 1mg/L 2,4-D and 0.00, 0.1, 1mg/L BAP) on callus induction and callus proliferation, as well as the effect of different imazamox (IMI) concentrations (control, 0.02, 0.04, 0.06, 0.08, and 0.1 M) on somatic embryogenesis and plant regeneration of cumin callus were evaluated. The result of the study showed that callus induction rate, callus proliferation rate, and plant regeneration from the callus tissue were significantly affected by the plant growth regulator and imazamox herbicide concentration. The highest values of callus induction rate (76.6%), callus proliferation (87.5%), and plant regeneration (45.8%) were obtained from 0.1mg/L BAP, 1mg/L BAP, and control treatments, respectively. The survival rate of callus tissue and plant regeneration was higher up to 0.06 µM imazamox treated medium, but after 0.06 µM imazamox no plant regeneration was observed. Therefore, 0.06 µM was recommended to get imazamox (IMI) resistant cumin plants. Further studies are needed to test the regenerated imazamox resistant plants under field conditions.
Keywords: Callus, Imazamox (IMI), Cumin, Cuminum cyminum, Herbicide resistant, Plant regeneration, Somatic embryogenesis